| 原文章发表时间:2010/12/3 |
| <标题>Domain closure in Venus7 ------------------------------------------------------ |
| <内容> Domain closure in Venus7 The description of domain closure using terminal block a single rotation angle is widely used to describe conformational perkins generator changes for iGluRs, mGluRs and PBPs, but has limits when making comparisons voltage regulator between structures for different proteins. A problem arises if the warning tape rotation movements underlying closure is associated with twisting motions along coach outlet store online another axis3. When the open cleft form of ugg nettbutikk mGluR1 (PDB 1EWK) is used for comparison with GluR6ATD the rotation angle moncler outlet is 30°, similar to value of 31.5° found when the open and closed forms moncler of mGluR1 (both PDB 1EWK) are compared, suggesting, incorrectly that the GluR6 ATD is closed to the same extent ugg cardyugg shoes as the glutamate bound complex of mGluR1. But, when the calculation is performed using the mGluR1 glutamate closed form as a reference ugg structure, we find that the GluR6 ATD is more open by 30°. Thus, the sum of the rotations for GluR6TAD moncler jacket compared to the mGluR1 open and online moncler shop closed reference structures is 63°, twice the value found moncler jassen when the open and closed structures of mGluR1 are compared. When ugg pas chers this series of structures are visually compared ugg australia by superposition of either domain 1 or domain 2 as shown in Fig. 4b there is no doubt that GluR6 ATD is indeed in an intermediate soldes ugg state, neither open nor closed, and that as a result the cleft between domains R1 ugg pas cher and R2 is open and exposed to solvent. For LIVBP, the difference is less moncler 2011 extreme, GluR6 ATD being 38° more moncler donna closed and 19° more open, compared to the moncler piumini apo and leucine bound complexes, for which moncler milano direct comparison reveals a 50° closure, compared to the 57° calculated from the sum for the GluR6ATD values. The R1R2 clam shell contains an ion moncler donna binding site We identified a putative negozio moncler cation binding site in GluR6 ATD in the loop between helix A moncler sito ufficiale and β strand 2, and refined a Ca2+ion in this position based on its moncler donna coordination and temperature factor (Supplementary cheap jackets Fig. 4). Strikingly, the mGluR1 crystal structure imitation louis vuitton also contains a bound ion at the equivalent position, and in both proteins the binding site is formed entirely by custom wedding dresses main chain carbonyl oxygen atoms4. In contrast, as a result of homecoming dresses a different loop conformation the ion binding site is absent in the GluR2 ATD crystal structure. The calcium ion is hexa-coordinated in one GluR6 runescape power leveling ATD protomer, where five of the interacting partners are the main chain carbonyls of Ile33, Asn34, Asn36, Leu39 and Leu40, with a water molecule in the 6th site. |
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